Actomyosin is the main driver of interkinetic nuclear migration in the retina

Norden c, young s, link ba, harris wa 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina. Interkinetic nuclear migration was first described in pig and chick neural tubes in 1935 sauer, 1935, and is conserved across vertebrates, insects and cnidaria meyer et al. Sep 18, 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina. This seems to be related to interkinetic nuclear migration, which is the apicobasal movement of the nucleus as the cell cycle progresses. Sep 15, 2018 analysis of interkinetic nuclear migration within retinal slice cultures as a first test of our protocol, we performed live imaging of rpc inm. Nuclear migration in mge neurons is similarly inhibited by blebbistatin treatment, suggesting that myosin activation at the rear is critical for nuclear translocation. Nuclei in the proliferative pseudostratified epithelia of vastly different organisms exhibit a characteristic dynamics the socalled interkinetic nuclear migration iknm. Pseudostratified epithelia pse are widespread and diverse tissue arrangements, and many pse are organ precursors in a variety of organisms. During interkinetic nuclear migration inm, the nuclei in many epithelial cells. Norden c, young s, link ba, harris wa 2009, actomyosin is the main driver of interkinetic nuclear migration in the. Interkinetic nuclear migration and the selection of neurogenic cell divisions during vertebrate retinogenesis. Actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden,1,3 stephen young,1,3 brian a.

Apical migration of nuclei during g2 is a prerequisite for all nuclear. Actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden 1,3, stephen young 1,3, brian a. Mar 22, 2011 read interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia, current biology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Apical migration of nuclei during g2 is a prerequisite for all nuclear motion in zebrafish neuroepithelia. Interkinetic nuclear migration in the regenerating retina. In addition, nuclei in pse undergo interkinetic nuclear migration iknm, also. Balanced organogenesis requires the orchestration of multiple cellular interactions to create the collective cell behaviours that progressively shape developing tissues. Interkinetic nuclear migration inm is an oscillatory nuclear movement that is synchronized with the progression of the cell cycle. While cells in pse, like other epithelial cells, feature apicobasal polarity, they generally are more elongated and their nuclei are more densely packed within the tissue.

Interkinetic nuclear migration and the selection of neurogenic cell. The efforts of several researchers, following the first report of inm in 1935, have revealed many of the molecular mechanisms of this fascinating phenomenon linking the timing of the cell cycle and nuclear positioning in tissue. Norden c, young s, link ba, harris wa 2009, actomyosin is the main driver of interkinetic nuclear migration in the retina. Frontiers mechanical regulation of nuclear translocation in. Culture of adult transgenic zebrafish retinal explants for livecell imaging by multiphoton microscopy. Sep 18, 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina author links open overlay panel caren norden 1 3 stephen young 1 3 brian a. Platelet activating factor blocks interkinetic nuclear. For example, both actomyosin and microtubules are required for cell division. Dipp dresden international phd program research research. Read interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia, current biology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Agathocleous m, harris wa 2009, from progenitors to differentiated cells in the vertebrate retina. The movie begins at 32 hpf and plays at seven frames per second at. Interkinetic nuclear migration is the process in which the nucleus migrates within the. Platelet activating factor blocks interkinetic nuclear migration in.

Pse undergo interkinetic nuclear migration iknm, also referred to as. Live imaging of developing mouse retinal slices neural. Agathocleous m, harris wa 2009, from progenitors to differentiated cells in the vertebrate retina, annu rev cell dev biol. We conclude that paf blocks interkinetic nuclear migration in retinal progenitor cells through an unusual arrest of the cell cycle at the transition from s to g2 phases. Apr 24, 2012 interkinetic nuclear migration is found in other epithelia undergoing morphogenetic movements beside the formation of hinge points. Statistical analyses in trials for the comprehensive. Sauer fc 1936 the interkinetic migration of embryonic epithelial nuclei. Actomyosin is the main driver of interkinetic nuclear migration in the retina previous article chl1 functions as a nitrate sensor in plants next article the antioxidant enzyme prdx1 controls neuronal differentiation by thiolredoxdependent activation of gde2. The retina as a model to explore the cell biology of morphogenesis from.

Movements of iknm are generally believed to involve smooth migration of. Quantitative analysis of growth and limits of growth in the developing neuroepithelium. Dscam and dscaml1 function in selfavoidance in multiple cell types in the developing mouse retina. Analysis of interkinetic nuclear migration within retinal slice cultures as a first test of our protocol, we performed live imaging of rpc inm.

These data suggest the operation, in the developing retina, of a checkpoint that monitors the transition from s to g2 phases of the cell cycle. Norden c, young s, link ba et al 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina. These nuclei move in a cell cycledependent manner, i. Actomyosin is the main driver of interkinetic nuclear migration in the retina author links open overlay panel caren norden 1 3 stephen young 1 3 brian a. Although these movements are thought to be intimately tied to the cell cycle, little is known about the relationship between iknm and distinct phases of the cell cycle and the role that this association plays in ensuring. Frontiers interkinetic nuclear migration generates and.

Actomyosin is the main driver of interkinetic nuclear migration in the. Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration iknm. Interkinetic nuclear migration during early development of. Nuclear migration and positioning are essential to a wide variety of developmental. Cellular systems for epithelial invagination philosophical.

Interkinetic nuclear migration inm during vertebrate neurogenesis is. Accumulation of actomyosin at the nuclear rear is also observed during migration. Caren norden, stephen young, brian a link, william a harris actomyosin is the main driver of interkinetic nuclear migration in the retina. Frontiers mechanical regulation of nuclear translocation. In vertebrates, interkinetic nuclear migration is crucial for the development of neuroepithelia, including the retina, neural tube and neocortex. Deconstruction of neuronal lamination in the retina at the cellular and tissue level over development. Professor william a harris, fmedsci frs department of.

Culture of adult transgenic zebrafish retinal explants for. Interkinetic nuclear migration in the mouse embryonic. Retinal neurogenesis begins with cell cycle exit and celltype. Apical movement during interkinetic nuclear migration is a twostep process apical movement during interkinetic nuclear migration is a twostep process spear, philip c erickson, carol a. Actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden, 1, 3 stephen young, 1, 3 brian a. Inm describes the periodic movement of the nucleus within a cell that occurs in phase with cell cycle progression and is a common feature of pseudostratified epithelia such as the developing retinal. Link is an urologist in achille, oklahoma and is affiliated with multiple hospitals in the area. One key manifestation of the ab polarity of rpcs is a process known as interkinetic nuclear migration iknm. Norden c, young s, link ba, harris wa, 2009, actomyosin is the main driver of interkinetic nuclear migration in the retina, cell, sep 18.

Adhesion sites in the rear need to be detached for cell migration. We conclude that paf blocks interkinetic nuclear migration in retinal. Caren norden department of physiology, development and neuroscience, cambridge university, cambridge, uk. Actomyosin is the main driver of interkinetic nuclear migration in the retinacell. Mar 14, 2012 interkinetic nuclear migration inm is an oscillatory nuclear movement that is synchronized with the progression of the cell cycle. Actomyosin is the main driver of interkinetic nuclear migration in the retina. Pdf actomyosin is the main driver of interkinetic nuclear. As the most accessible part of the vertebrate central nervous system cns, the neural retina nr is an excellent system in which to analyze key aspects of neurogenesis, particularly since both the retinal architecture and the mechanisms underlying retinal development are highly conserved across vertebrates. Interkinetic nuclear migration generates and opposes. Apical movement during interkinetic nuclear migration is a. This behavior is observed in both chicken neuraltubeslice preparations and in mouse cortical slices, and therefore is likely to be a general feature of interkinetic nuclear migration. Harris cell, volume 8, issue 6, 18 september 2009, pages 11951208. Interkinetic nuclear migration in the zebrash retina as a.

Sep 18, 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden, 1, 3 stephen young, 1, 3 brian a. Mechanical properties of surrounding tissues also contribute to interkinetic nuclear migration in neuroepithelial. Movements of iknm are generally believed to involve smooth migration of nuclei from apical to basal and back during the g1 and g2 phases of the cell cycle, respectively. Live imaging of whole mouse embryos during gastrulation. Staggered cellintrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina. Shinoda t and kawaguchi a 2015 interkinetic nuclear migration generates and. Nuclear migration events throughout development journal of. One key cellular decision, which is essential for appropriate numbers and. Actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden1,3, stephen young1,3, brian a. Actomyosin is the main driver of interkinetic nuclear migration in the retina caren norden, stephen young, brian a link, william a. Nuclear locations were sampled at 2 or 5 min intervals at different phases of the cell cycle.

Interkinetic nuclear migration inm is a phenomenon in which progenitor cell nuclei migrate along the apico. Interkinetic nuclear movements promote apical expansion in. Interkinetic nuclear migration generates and opposes ventricularzone crowding. Jan 28, 2015 in the nevz, neural progenitor cells are elongated along the apicobasal axis, and their nuclei assume different apicobasal positions. Sep 18, 2009 actomyosin is the main driver of interkinetic nuclear migration in the retina previous article chl1 functions as a nitrate sensor in plants next article the antioxidant enzyme prdx1 controls neuronal differentiation by thiolredoxdependent activation of gde2. Selforganizing opticcup morphogenesis in threedimensional. Actomyosin is the main driver of interkinetic nuclear migration in the retina, caren norden, stephen young, brian a. Representative in vivo confocal timelapse movie of interkinetic nuclear migration in a control retinal neuroepithelium.

Research groups dipp dresden international phd program. Kif1a inhibition immortalizes brain stem cells but blocks. In the zebrafish retina, myosin inhibition with blebbistatin also stops apical. Nuclear migration events throughout development journal of cell. Actincytoskeleton and rockmediated inm are required for. Harris1, 1department of physiology, development and neuroscience, cambridge university, downing street, cambridge cb2 3dy, uk 2department of cell biology, neurobiology, and anatomy, medical college of wisconsin, 8701 watertown plank road. How does actomyosin contractility at the rear of the cell drive nuclear transport in these cells. Cell, 86 11951208 2009 manuel mendoza, caren norden, kathrin durrer, harald rauter, frank uhlmann, yves barral a mechanism for chromosome segregation sensing by the nocut checkpoint. Ne of the zebrafish retina and hindbrain leung et al. Kinesin 3 and cytoplasmic dynein mediate interkinetic nuclear. Apical migration of nuclei during g2 is a prerequisite for. Actomyosin is the main driver of interkinetic nuclear.

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